Search
Latest topics
AGGLUTINATION REACTION
Page 1 of 1
AGGLUTINATION REACTION
Khadija Batool 2206M010
AGGLUTINATION REACTION
When a particulate antigen is mixed with its antibody in the presence of electrolytes at a suitable temperature and pH, the particles are clumped or agglutinated due to lattice formation. Agglutination reaction is more sensitive than precipitation reactions for the detection of antibodies. The same principles govern agglutination and precipitation. Incomplete or monovalent antibodies do not cause agglutination, though they combine with the antigen. They may act as blocking antibodies, inhibiting agglutination by the complete antibody added subsequently.
Excess of antibody leads to prozone and excess of antigen leads to postzone phenomenon.
Types of agglutination:-
1. SLIDE AGGLUTINATION:-
a. Drop of antiserum + a drop of uniform suspension of antigen clump formation
b. Control is a must
c. Visible to the naked eye
d. Uses - blood grouping , serotyping of bacteria
2. TUBE AGGLUTINATION:-
a. Standard quantitative method for the measurement of antibodies
b. Uses WIDAL TEST for typhoid fever
c. Heterophile agglutination test : Weil-Felix
d. Haemagglutination- RBCs are used as antigens
e. Example Paul - Bunell test , Cold agglutination test
3. PASSIVE AGGLUTINATION:-
a. Soluble antigens are attached to the surface of the carrier particles to convert precipitation tests into agglutination tests.
b. More convenient and sensitive
c. Carrier particles-RBCs , Latex, Bentonite
d. Example-ASO test
AGGLUTINATION REACTION
When a particulate antigen is mixed with its antibody in the presence of electrolytes at a suitable temperature and pH, the particles are clumped or agglutinated due to lattice formation. Agglutination reaction is more sensitive than precipitation reactions for the detection of antibodies. The same principles govern agglutination and precipitation. Incomplete or monovalent antibodies do not cause agglutination, though they combine with the antigen. They may act as blocking antibodies, inhibiting agglutination by the complete antibody added subsequently.
Excess of antibody leads to prozone and excess of antigen leads to postzone phenomenon.
Types of agglutination:-
1. SLIDE AGGLUTINATION:-
a. Drop of antiserum + a drop of uniform suspension of antigen clump formation
b. Control is a must
c. Visible to the naked eye
d. Uses - blood grouping , serotyping of bacteria
2. TUBE AGGLUTINATION:-
a. Standard quantitative method for the measurement of antibodies
b. Uses WIDAL TEST for typhoid fever
c. Heterophile agglutination test : Weil-Felix
d. Haemagglutination- RBCs are used as antigens
e. Example Paul - Bunell test , Cold agglutination test
3. PASSIVE AGGLUTINATION:-
a. Soluble antigens are attached to the surface of the carrier particles to convert precipitation tests into agglutination tests.
b. More convenient and sensitive
c. Carrier particles-RBCs , Latex, Bentonite
d. Example-ASO test
Page 1 of 1
Permissions in this forum:
You cannot reply to topics in this forum
|
|
Wed Oct 22, 2014 6:51 am by Johnsnow
» protozoa inactivation
Wed Jun 29, 2011 4:28 pm by Guest
» Contributions of louis pasteur
Sun Jun 07, 2009 6:52 pm by arsham
» Immuno prophylaxis of tetanus
Sat Jun 06, 2009 11:16 pm by sakia_fazal
» pathogenecity of CLOSTRIDIUM PERFINGES
Sat Jun 06, 2009 11:03 pm by sakia_fazal
» naglers reaction
Sat Jun 06, 2009 10:53 pm by sakia_fazal
» LABORATORY DAIGNOSIS OF SYPHILIS
Sat Jun 06, 2009 9:27 pm by sakia_fazal
» Trichomonas vaginalis
Sat Jun 06, 2009 6:31 pm by sakia_fazal
» instestinal ameobiasis
Sat Jun 06, 2009 4:49 pm by sakia_fazal