Display results as :

Rechercher Advanced Search

Latest topics
» Virology MCQs 1-25
Wed Oct 22, 2014 6:51 am by Johnsnow

» protozoa inactivation
Wed Jun 29, 2011 4:28 pm by Guest

» Contributions of louis pasteur
Sun Jun 07, 2009 6:52 pm by arsham

» Immuno prophylaxis of tetanus
Sat Jun 06, 2009 11:16 pm by sakia_fazal

» pathogenecity of CLOSTRIDIUM PERFINGES
Sat Jun 06, 2009 11:03 pm by sakia_fazal

» naglers reaction
Sat Jun 06, 2009 10:53 pm by sakia_fazal

Sat Jun 06, 2009 9:27 pm by sakia_fazal

» Trichomonas vaginalis
Sat Jun 06, 2009 6:31 pm by sakia_fazal

» instestinal ameobiasis
Sat Jun 06, 2009 4:49 pm by sakia_fazal


free forum

SPECIMEN ANSWER TO SAQ – Autoclave by Dr. Sudheer Kher

Go down

SPECIMEN ANSWER TO SAQ – Autoclave by Dr. Sudheer Kher

Post  sudheerkher on Wed Nov 12, 2008 9:06 pm


Principle- Physical method of sterilization by using Steam under pressure.
Advantages of steam – High penetration, Latent heat.
Temp & Time – 121 C X 20 min achieved by 15 lb/sq inch
Mode of destruction – Coagulative denaturation.
Types of autoclaves – Jacketed and non-jacketed.
Problems of non jacketed
1. Air discharge not satisfactory
2. Load remains moist at the end.
Jacketed autoclave – Both the above problems are taken care of by
a) Having air discharge at the bottom
b) Separate circulation of steam within the jacket after holding period allows drying of the load.

Articles sterilised – Most culture media (barring those containing blood/ serum/ eggs/sugars), dressings & bandages, bowls, instruments, syringes, glassware, glass and metal syringes, filters, rubber goods etc.

Articles not sterilised – Talc, Oils, Greases and Fats. Blood/serum/egg/sugar containing media.

Autoclave efficiency tests
a) Physical – Thermocouple, Recording drums, thermometers
b) Chemical – Bowie-Dick tapes, Browne’s tubes
c) Biological – Using B. stearothermophilus spores


Posts : 65
Join date : 2008-11-12
Age : 70
Location : Ajman, UAE

View user profile

Back to top Go down

Back to top

- Similar topics

Permissions in this forum:
You cannot reply to topics in this forum